Preparation and Quality Evaluation of Scutellarin-PEG-PLGA Drug-loaded Nanoparticles
10.6039/j.issn.1001-0408.2015.19.35
- VernacularTitle:灯盏乙素-PEG-PLGA载药纳米粒的制备及质量评价
- Author:
Hongbi ZU
;
Xiaohua LI
- Publication Type:Journal Article
- Keywords:
Scutellarin;
PEG-PLGA;
Nanoparticle;
Encapsulation efficiency;
Particle size
- From:
China Pharmacy
2015;(19):2705-2707,2708
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To prepare the scutellarin-PEG-PLGA drug-laded nanoparticles,optimize the formulation with ratio of colostrum and external aqueous phase,mass concentration of scutellarin and PEG-PLGA as factors and conduct quality evalua-tion. METHODS:A double emulsion-solvent evaporation method was adopted to prepare the scutellarin-PEG-PLGA nanoparticles. With the evaluation index of encapsulation efficiency,single factor and orthogonal test were used to determine the apparent shape, particle size,Zeta potential,drug loading amount,encapsulation efficiency and stability of prepared nanoparticles by optimized for-mulation with ratio of colostrum and external aqueous phase,mass concentration of scutellarin and PEG-PLGA as factors. RE-SULTS:The ratio of colostrum and external aqueous phase was 1∶15 and the mass concentrations of scutellarin and PEG-PLGA were respectively 10 mg/ml and 15 mg/ml in optimized formulation;the prepared nanoparticles were round or oval with the average particle size of(78.54±2.21)nm,Zeta potential of(-23.07±1.39)mV,drug loading amount of(1.67±0.12)%,and encapsula-tion efficiency of(45.32±1.29)%. There was no obvious changes in the particle size and encapsulation efficiency within 3 months at 4 ℃ preservation. CONCLUSIONS:The scutellarin-PEG-PLGA nanoparticles with better physical and chemical properties and stability are successfully prepared.
