Content Determination of Berberine Hydrochloride in Jiangtang Xiaozhi Tablets by HPLC
10.6039/j.issn.1001-0408.2015.18.28
- VernacularTitle:HPLC法测定降糖消脂片中盐酸小檗碱的含量
- Author:
Ye REN
;
Hui XU
;
Zhengyan GE
;
Long JIN
;
Jianxun LIU
;
Yujie GUO
- Publication Type:Journal Article
- Keywords:
Jiangtang xiaozhi tablets;
Berberine hydrochloride;
HPLC;
Content determination
- From:
China Pharmacy
2015;(18):2530-2531,2532
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of berberine hydrochloride in the Jiangtang xiao-zhi tablets. METHODS:HPLC was conducted with the Symmetry C18 column. The mobile phase was acetonitrile-0.05 mol/L sodium dihydrogen phosphate(pH adjusted to 3.0 using phosphoric acid)(24∶76,V/V)at the flow rate of 1.0 ml/min. The detection wave-length was 345 nm,the column temperature was room temperature and injection volume was 10 μl. RESULTS:The linear range of berberine hydrochloride was 0.522-4.698 μg(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 0.72%;the average recovery was 97.79%(RSD=2.09%,n=6). CONCLUSIONS:The method is simple,accurate,sensitive and reproducible,and can be used for the content determination of berberine hydrochloride.
