Content Determination of the 2 Lignan Components in Mongolian Medicine Compound Nutmeg-5 by HPLC
10.6039/j.issn.1001-0408.2015.18.31
- VernacularTitle:HPLC法同时测定蒙药复方肉豆蔻五味中2种木脂素类成分的含量
- Author:
Xiaoling ZHU
;
Yu DONG
;
Guoyuan SUN
- Publication Type:Journal Article
- Keywords:
Nutmeg-5;
Dehydrodiisoeugenol;
2,3-dihydro-7-methoxy-2-(3,4-methylenedioxyphenyl)-3-methyl-5-(E)-prope-nyl-benzofuran;
HPLC
- From:
China Pharmacy
2015;(18):2538-2540,2541
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the determining contents of 2 lignan components[dehydrodiisoeugenol and 2,3-dihydro-7-methoxy-2-(3,4-methylened ioxyphenyl)-3-methyl-5-(E)-propenyl-benzofuran(referred to“lignanoid 2”)]. METH-ODS:HPLC method was adopted. The column was Elite C18 with the mobile phase of water-methanol(gradient elution)at the flow rate of 1.0 ml/min;the detection wavelength was 225 nm and the column temperature was 30 ℃. The sample size was 20 μl. RE-SULTS:There was a good linear relationship between sample quantity and the peak area in the range of 0.202-2.02 μg(r=0.999 9) and 0.204-2.04 μg(r=0.999 9)for 2 lignan components. The RSD of precision,stability and repeatability tests were less than 2%with the average recovery of 101.54%(RSD=0.60%,n=6)and 99.43%(RSD=1.09%,n=6). CONCLUSIONS:The method is simple,sensitive and accurate,and can be used for the quantization determination of dehydrodiisoeugenol and lignanoid 2 in nut-meg-5.