Content Determination of Chloramphenicol and Salicylic Acid in Acne Tincture by HPLC
10.6039/j.issn.1001-0408.2015.18.38
- VernacularTitle:HPLC法测定痤疮酊中氯霉素和水杨酸的含量
- Author:
Liang TENG
;
Quanmei ZHANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Acne tincture;
Chloramphenicol;
Salicylic acid;
Content determination
- From:
China Pharmacy
2015;(18):2557-2558,2559
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of chloramphenicol and salicylic acid in Acne tinc-ture. METHODS:HPLC method was adopted with the column of Shim-pack CLC-ODS with the mobile phase of methanol-water (50∶50,V/V)at the flow rate of 0.8 ml/min. The detection wavelength was 278 nm,column temperature was 35 ℃,and the vol-ume was 10 μl. RESULTS:The linear range was 2.0-10.0 μg/ml(r=0.999 2)for chloramphenicol and 5.0-25.0 μg/ml(r=0.999 1) for salicylic acid;the RSDs of precision,stability and repeatability tests were lower than 1%;the average recoveries chlorampheni-col and salicylic acid were respectively 99.77%(RSD=0.35%,n=9) and 99.29%(RSD=0.48%,n=9). CONCLUSIONS:The method is simple,rapid,accurate,specific,precise,and can be used for quality control of Acne tincture.
