Determination of the Main Content and Entrapment Efficiency in the Brucine Nanostructured Lipid Carrier by HPLC

VernacularTitle:HPLC法测定马钱子碱纳米结构脂质载体的主药含量及包封率Δ
Author: Qingxia GUAN ; Xiaodan HUA ; Xiwu ZHANG ; Qiuhong LI ; Weinan LI ; Yuwei ZHAO
Publication Type:Journal Article
Keywords: Brucine; Nanostructured lipid carrier; HPLC; Sephadex gel filtration method; Content; Entrapment efficiency
From: China Pharmacy 2015;(21):2983-2985,2986
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To establish a method for determining the main contents and entrapment efficiency in the bruc-ine nanostructured lipid carrier(NLC). METHODS:HPLC was adopted to determine the main content,sephadex gel filtration meth-od was employed to separate free drug in brucine NLC to determine the entrapment efficiency. The column was Dikma C18 with the mobile phase of mobile phase A(methanol)-mobile phase B [water-acetic acid-triethylamine(230∶2.4∶0.3,V/V/V)](30∶70,V/V)at the flow rate of 1 ml/min,the detection wavelength was 265 nm,volume was 10 μl and temperature was 30 ℃. RESULTS:The linear range of brucine was 4.00-80.00μg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were≤1.67%;av-erage recoveries of content determination and sephadex gel filtration method were respectively 99.66%(RSD=0.45%,n=9) and 99.75%(RSD=1.74%,n=9);and the average entrapment efficiency was 69.92%. CONCLUSIONS:The method is simple,re-producible and efficient,and can be used for the determination of main contents and entrapment efficiency in brucine NLC.