Content Determination of Ephedrine Hydrochloride in Luofu Mountain Rheumatism Plaster by HPLC
10.6039/j.issn.1001-0408.2015.21.41
- VernacularTitle:HPLC法测定罗浮山风湿膏药中盐酸麻黄碱的含量
- Author:
Jiabin YANG
;
Xianping ZHANG
;
Shiping WU
;
Shaozhi GONG
- Publication Type:Journal Article
- Keywords:
Luofu mountain rheumatism plaster;
Ephedrine hydrochloride;
HPLC;
Content determination
- From:
China Pharmacy
2015;(21):2994-2995,2996
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of ephedrine hydrochloride in Luofu mountain rheumatism plaster. METHODS:HPLC was performed on the column of Eclipse XDB-C18 with the mobile phase of acetoni-trile-0.1%phosphoric acid(9∶91,V/V),at the flow rate of 1.0 ml/min. The detection wavelength was 207 nm,the column tempera-ture was 40 ℃,and the volume was 5 μl. RESULTS:The linear range of ephedrine hydrochloride was 81.6-408 ng(r=0.999 7);RSDs of precision,stability and repeatability tests were lower than 2%;and the average recovery rate was 96.73%(RSD=1.9%, n=6). CONCLUSIONS:The method is fast and accurate,and can be used for the content determination of ephedrine hydrochlo-ride in Luofu mountain rheumatism plaster.
