Content Determination of Ferulic Acid in Tianwang Buxin Pills by HPLC
10.6039/j.issn.1001-0408.2015.21.45
- VernacularTitle:HPLC法测定天王补心丸中阿魏酸的含量
- Author:
Wangpei LIU
;
Yichun HUANG
;
Hualong CHEN
- Publication Type:Journal Article
- Keywords:
HPLC;
Tianwang buxin pill;
Ferulic acid;
Content determination
- From:
China Pharmacy
2015;(21):3003-3004,3005
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method to determine the content of ferulic acid in Tianwang buxin pills. METHODS:HPLC was performed on the column of Diamonsil C18 with the mobile phase of acetonitrile-1% glacial acetic acid solution(15∶85, V/V)at the flow rate of 1.0 ml/min,the detection wavelength was 323 nm,the temperature was room temperature,and the volume was 20 μl. RESULTS:The linear range of ferulic acid was in the range of 0.091 2-2.28 μg/ml(r=0.999 8);the RSDs of precision, stability and reproducibility tests were less than 2.0%,and the average recovery was 98.96%(RSD=0.26%,n=9). CONCLU-SIONS:The method is simple,accurate and reliable,and can be used as the quality control method of ferulic acid in Tianwang buxin pills.
