Content Determination of Phosphatidylcholine in Eustachian Tube Lavage Fluid of Patients with Secretory Otitis Media by HPLC
10.6039/j.issn.1001-0408.2015.21.39
- VernacularTitle:HPLC法测定分泌性中耳炎患者咽鼓管灌洗液中磷脂酰胆碱的含量Δ
- Author:
Ling XIE
;
Hua XIE
;
Daihua LIU
;
Yu QIN
;
Jiancheng LIANG
- Publication Type:Journal Article
- Keywords:
Secretory otitis media;
Eustachian tube lavage fluid;
Phosphatidylcholine;
Content determination
- From:
China Pharmacy
2015;(21):2989-2990,2991
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for content determination of phosphatidylcholine in eustachian tube lavage fluid of patients with secretory otitis media. METHODS:HPLC was used. The samples were pretreated by liquid-liquid extraction. It was performed on the column of Hypersil CN with mobile phase of acetoneitril-methanol-phosphoric acid (100∶10∶0.6,V/V/V)at the flow rate of 1.8 ml/min,the detection wavelength was 205 nm,temperature was 30 ℃ and volume was 20 μl. RESULTS:The lin-ear range of phosphatidylcholine was 11.99-119.9 μg/ml(r=0.999 6);RSDs of precision tests of intra-day and inter-day were no more than 15%;average recovery was 97.54%(RSD=9.36%,n=9);the average content of phosphatidylcholine in eustachian tube lavage fluid of patients was(24.43±3.61)μg/ml. CONCLUSIONS:The method is simple and accurate,and can be used for the content determination of phosphatidylcholine in eustachian tube lavage fluid of patients with secretory otitis media.
