Simultaneous Contents Determination of Schizandrin,Schizandrin B and Cinnamaldehyde in Xiaoqinglong Granule by HPLC
10.6039/j.issn.1001-0408.2015.21.44
- VernacularTitle:HPLC法同时测定小青龙颗粒中五味子醇甲、五味子乙素及桂皮醛的含量
- Author:
Zongwei LI
;
Jianming WANG
- Publication Type:Journal Article
- Keywords:
Xiaoqinglong granule;
HPLC;
Schizandrin;
Schizandrin B;
Cinnamaldehyde
- From:
China Pharmacy
2015;(21):3001-3002,3003
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous contents determination of schizandrin,schizandrin B and cin-namaldehyde in Xiaoqinglong granule. METHODS:HPLC was performed on the column of Wondasil-C18 with the mobile phase of acetonitrile-0.1% phosphoric acid solution(gradient elution)at the flow rate of 1.0 ml/min,the detection wavelength was 245 nm, temperature was 25 ℃ and volume was 20 μl. RESULTS:The linear range was 1.008-20.16 μg/ml(r=0.999 8) for schizandrin, 0.496-9.92μg/ml(r=0.999 7)for schizandrin B,and 1.012-20.24μg/ml(r=0.999 6)for cinnamaldehyde;RSDs of precision,sta-bility and reproducibility tests were no more than 1.33%;the average recoveries were respectively 98.9%(RSD=1.71%,n=6), 99.9%(RSD=1.50%,n=6) and 98.7%(RSD=2.10%,n=6). CONCLUSIONS:The method is simple,accurate and reproduc-ible,and can be used for the quality control of Xiaoqinglong granule.
