DNA Extraction of Cast-off Cells of Fingerprints from 502 Glue Fumigated Con-tact Samples
10.3969/j.issn.1004-5619.2015.06.010
- VernacularTitle:接触性检材502胶熏显后手印脱落细胞的DNA提取
- Author:
Xianwen WANG
;
Xuefeng LENG
;
Shouyu WANG
- Publication Type:Journal Article
- Keywords:
forensic genetics;
DNA;
cells;
acetone;
fingerprint;
adhesives
- From:Journal of Forensic Medicine
2015;(6):454-455,461
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of fingerprint position, sample transfer and fingerprint DNA extraction in contact samples. Methods Sixty-six cases were visualized by 502 glue fingerprint fumiga-tion. Two methods, ordinary wipe and acetone wipe, were used to transfer cast-off cells of fingerprints fromtesting samples, respectively. DNA was extracted and purified by ultramicro magnetic bead kit. The data was resolved on genetic analysis after amplification. Results In 33 samples, 30 samples got better STR analysis by acetone wipe method. The peak range was 1 000-4 000 R FU and peak shapes were equable. It was hard to get ideal STR typing by ordinary wipe method. Conclusion The samples are visualized by 502 glue fingerprint fumigation and the case-off cells are transferred by acetone wipe method. The method shows better STR analysis result, which might be a better method for forensic sci-ence practice.
