Development of a Forensic Multiplex Amplification STR Kit for 15 Autosomal STR Loci and 10 Y-STR Loci

VernacularTitle:15个常染色体和10个Y-STR基因座复合扩增试剂盒的研发
Author: Yan DONG ; Shuangshuang LIN ; Yu CAO ; Weiwei WU ; Shuqin HUANG ; Weiguo ZHENG ; Fayuan LI ; Binwen GE ; Yulin GUO ; Huaigu ZHOU
Publication Type:Journal Article
Keywords: forensic genetics; reagent kits; short tandem repeat sequences; autosom e; Y chrom osom e
From: Journal of Forensic Medicine 2015;(5):373-376,380
CountryChina
Language:Chinese
Abstract: Objective To establisha multiplex STR genotyping m ethod for autosom al STR and Y-STR loci in forensic biological practice. Methods W idely used autosom al STR loci and Y-STR loci w ere se-lected. A set of PC R prim ers w as designed, and a 5-dye fluorescent labeled STR multiplex PC R reagent kit w as developed. Results A kit w as developed w hich can sim ultaneously detect 15 autosom al STR loci, 10 Y-STR loci, and an Amelogenin. Conclusion The 15 autosom al STR plus 10 Y-STR kit in com bination w ith capillary electrophoresis m ethod w as used to STR genotyping w ith accurate and reli-able results. The new one-step testing kit can potentially be w idely used in forensic cases and D N A databank in the future.