Development of a Forensic Multiplex Amplification STR Kit for 15 Autosomal STR Loci and 10 Y-STR Loci
10.3969/j.issn.1004-5619.2015.05.011
- VernacularTitle:15个常染色体和10个Y-STR基因座复合扩增试剂盒的研发
- Author:
Yan DONG
;
Shuangshuang LIN
;
Yu CAO
;
Weiwei WU
;
Shuqin HUANG
;
Weiguo ZHENG
;
Fayuan LI
;
Binwen GE
;
Yulin GUO
;
Huaigu ZHOU
- Publication Type:Journal Article
- Keywords:
forensic genetics;
reagent kits;
short tandem repeat sequences;
autosom e;
Y chrom osom e
- From:
Journal of Forensic Medicine
2015;(5):373-376,380
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establisha multiplex STR genotyping m ethod for autosom al STR and Y-STR loci in forensic biological practice. Methods W idely used autosom al STR loci and Y-STR loci w ere se-lected. A set of PC R prim ers w as designed, and a 5-dye fluorescent labeled STR multiplex PC R reagent kit w as developed. Results A kit w as developed w hich can sim ultaneously detect 15 autosom al STR loci, 10 Y-STR loci, and an Amelogenin. Conclusion The 15 autosom al STR plus 10 Y-STR kit in com bination w ith capillary electrophoresis m ethod w as used to STR genotyping w ith accurate and reli-able results. The new one-step testing kit can potentially be w idely used in forensic cases and D N A databank in the future.