LATS1-YAP pathway regulate proliferation of human skin fibroblast and synthesis of extracellular matrix
10.11659/jjssx.1672-5042.201401010
- VernacularTitle:LATS1-YAP信号通路对人皮肤成纤维细胞增殖及细胞外基质合成的调控
- Author:
Hao LU
;
Ting LIU
;
Yu CHEN
;
Tongchun MAO
;
Zeyuan LEI
;
Dongli FAN
- Publication Type:Journal Article
- Keywords:
human skin fibroblast;
LATS1-YAP pathway;
proliferation;
extracellular matrix
- From:
Journal of Regional Anatomy and Operative Surgery
2014;(1):27-29,33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the proliferation of human skin fibroblast and synthesis of extracellular matrix which were regulated by LATS1-YAP pathway. Methods They were divided into three groups:control groups, LATS1 siRNA intervention group and YAP siRNA treatment group. Using LATS1 siRNA transferred human skin fibroblasts cell lines HS27 in LATS1 siRNA intervention group, and using YAP siRNA transferred HS27 in YAP siRNA treatment group. Expression of LATS1,YAP and collageⅠwere detected by western-blot 48 h later, and the activity of HS27 cells was determined by MTT. Results Compared with control group,expression of LATS1 protein decreased while expression of YAP protein and collagenⅠprotein increased 48 h after LATS1 siRNA transfection. Expression of LATS1 protein remains un-changed and expression of YAP protein and collagenⅠprotein decreased 48 h after YAP siRNA transfection. Conclusion LATS1-YAP pathway could regulate proliferation of human skin fibroblast and synthesis of extracellular matrix. It provides a potential therapeutic targets for skin wound repair and cicatrization.
