Inhibitory effect of interferonα-1b and cyclooxygenase-2 inhibitor on renal cancer cell and xenografts in nude mouse
10.11659/jjssx.12E015143
- VernacularTitle:干扰素α-1b联合环氧化酶-2抑制剂对肾癌细胞及其裸鼠移植瘤的抑制作用
- Author:
Liubing XIA
;
Zhou LONG
;
Teng ZHANG
;
Xingyou DONG
;
Jia LI
;
Chao WU
;
Qian LIU
;
Bo SONG
;
Longkun LI
- Publication Type:Journal Article
- Keywords:
renal cell carcinoma;
cyclooxygenase-2 inhibitor;
interferonα-1b;
ACHN cell;
nude mouse;
chemotherapy
- From:
Journal of Regional Anatomy and Operative Surgery
2016;25(3):157-161,162
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect on human ACHN cell line and its mice xenograft by using interferon α-1b combined with cyclooxygenase-2 inhibitor and the relevant mechanism in vitro and vivo experiment .Methods ACHN cell and the xenograft mice were devided into 4 groups(IFN-α1b,NS398,IFNα-1b+NS398 and control group).The inhibitory effects were tested by CCK8(Cell Counting Kit 8)assay after AHCN were treated for 24 h and 48 h.The expression of bcl-xl and COX-2 were detected by Western blot .The vol-ume of the xenografts of ACHN cell line and testing the expression of VEGF in xenografts were measured by immunohistochemistry assay .Re-sults Both IFNα-1b and NS398 exerted inhibitory effects on ACHN and this effects showed a rising trend with a increasing concentration of drugs.The combined group was more significant than monotherapy group (P<0.05).Western blot assay showed that IFNα-1b and NS398 downregulated the expression of bcl-xl and COX-2 in ACHN.The combined group was more significant than monotherapy group (P<0.05). The combined group has the greatest inhibitory effects on the xenografts of ACHN cell line compared with monotherapy group and control group(P<0.05).The expression of VEGF in tumor was obiviously inhibited in combined group compared with monotherapy group and con -trol group (P<0.05).Conclusion IFNα-1b combined with NS398 can inhibit the proliferation of ACHN and suppress the tumor growth .
