Experimental research on distribution of 125 I labeled recombinant fusion protein dTMP-GH in mice

VernacularTitle:125 I标记重组融合蛋白dTMP-GH在小鼠体内分布的实验研究
Author: Mingqiang SHEN ; Mo CHEN ; Song WANG ; Yongping SU ; Junping WANG
Publication Type:Journal Article
Keywords: 125 I labeled; dTMP-GH; blood platelet; mice
From:Journal of Regional Anatomy and Operative Surgery 2014;(3):221-223,224
CountryChina
Language:Chinese
Abstract: Objective To assess the distribution of recombinant fusion protein dTMP-GH in mice and to determine whether it is of tar-geted distribution characteristics. Methods A laboratory scale preparation of dTMP-GH recombinant fusion protein was obtained. Protein dT-MP-GH was labeled with radioactive 125 I,then mice were sacrificed at 5 min,15 min,30 min,1 h,2 h,4 h,8 h,12 h,24 h after tail vein injec-tion of 125 I-dTMP-GH at a dose of 100 μg/kg,and the organs and tissues ( heart,liver,spleen,kidney,bone and thyroid) were collected for radioactive counting. Results Preparation of purified ( >98%) dTMP-G was obtained. 125 I labeling rate was 71. 53%,radiochemical purity was 96. 53%,and specific activity was 0. 22 MBq/μl. 30 min after tail vein injection of 125 I labeled dTMP-GH,radioactivity accounted for 10% of the total injected in femoral,and metabolism was carried via liver and kidney over time. Conclusion Fusion protein mainly distribu-ted in bone marrow via tail vein injection in mice,which expressed that dTMP-GH has the characteristics of selective distribution in bone mar-row tissue.