Ultrastructural changes and HSP70 expression in mice liver after microwave irradiation with lethal dose
10.11659/jjssx.03E016063
- VernacularTitle:致死剂量微波辐照后小鼠肝超微结构及HSP70表达的变化
- Author:
Xiaohua WANG
;
Mingshu LIU
;
Shufang LIU
;
Yingxue HUANG
;
Ruichun WANG
;
Wei JIANG
;
Zao YANG
;
Hongji QI
;
Zhou ZHOU
;
Zhengping YU
- Publication Type:Journal Article
- Keywords:
microwave;
lethal dose;
liver;
HSP70
- From:Journal of Regional Anatomy and Operative Surgery
2016;25(8):555-557,558
- CountryChina
- Language:Chinese
-
Abstract:
Astract:Objective To investigate the ultrastructural changes and HSP70 expression in liver of mice after microwave irradiation with lethal dose and to explore the application of these indexes as the basis of medical identification in microwave irradiation induced death. Methods The mice were divided into the control group and the irradiation group. Mice of the irradiation group were induced death by whole body exposure to 129 W/cm2 microwave irradiation for 30 minutes. The ultrastructure of liver was observed by transmission electron micro-scope;changes of the HSP70 mRNA and protein expression in liver were detected by reverse transcription polymerase chain reaction ( RT-PCR) and Western blotting respectively. Results Liver cytoplasm was observed dissolved with points and sheets and there were mitochondri-al crest and membrane solution in the irradiation group. And the HSP70 mRNA and protein expression level increases significantly compared with the control group with statistically significant difference (P<0. 01). Conclusion Death induced by microwave irradiation could lead to liver cytoplasm dissolution, mitochondria damage, mRNA and protein expression of HSP70 up-regulation, which may be used as important diagnostic indicators of microwave irradiation induced death.
