Application of Sperm Selection Using Hyaluronic Acid Binding in Intracytoplasmic Sperm Injection Cycles: A Sibling Oocyte Study.
10.3346/jkms.2012.27.12.1569
- Author:
Seung Ah CHOE
1
;
Jin Chul TAE
;
Mi Young SHIN
;
Hyun Jung KIM
;
Chung Hyon KIM
;
Joong Yeup LEE
;
Doyeong HWANG
;
Ki Chul KIM
;
Chang Suk SUH
;
Byung Chul JEE
Author Information
1. Institute of Fertility and Genetics, Hamchoon Women's Clinic, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't ; Randomized Controlled Trial
- Keywords:
Hyaluronic Acid;
Sperm Selection;
Intracytoplasmic Sperm Injection
- MeSH:
Adult;
Blastocyst/cytology;
Embryo Transfer;
Female;
*Fertilization in Vitro;
Humans;
Hyaluronic Acid/*pharmacology;
Infertility, Male/therapy;
Male;
Oocytes/cytology/physiology;
Pregnancy;
Pregnancy Rate;
Prospective Studies;
*Sperm Injections, Intracytoplasmic;
Spermatozoa/*drug effects/physiology
- From:Journal of Korean Medical Science
2012;27(12):1569-1573
- CountryRepublic of Korea
- Language:English
-
Abstract:
The purpose of this study was to investigate whether sperm selection by hyaluronic acid (HA) binding could improve fertilization rate and embryo quality in intracytoplasmic sperm injection (ICSI) cycles. Two hundred nineteen oocytes obtained from eighteen women were injected with either HA-bound (n = 107) or conventionally selected spermatozoa (n = 112) in a randomized way. All of the participants were infertile couples who had normal sperm parameters but low fertilization rate in previous in vitro fertilization (IVF) cycle (n = 5) or experienced multiple IVF failures (n = 13). Lower fertilization (75.7% vs 83.0%) and cleavage rate on day 2 (72.9% vs 83.0%) was observed in oocytes injected with HA-bound spermatozoa than the conventional group, but the difference was not significant. Significantly lower cleavage rate was observed on day 3 in HA group (56.0% vs 69.6%, P = 0.038). Blastocyst formation rate and the number of transferred embryos were similar in both groups. In multiple IVF failure patients, significantly reduced fertilization rate (71.8% vs 85.3%, P = 0.046) and cleavage rate on day 2 (70.4% vs 85.3%, P = 0.029) and day 3 (53.5% vs 77.3%, P = 0.002) were noticed in HA group. Five women achieved pregnancy continuing more than 12 weeks after transfer (27.8%). Success of ICSI was not related with the number of embryos fertilized by HA-bound spermatozoa. Application of ICSI by sperm selection using HA binding is not helpful in couples with repeated poor fertilization or implantation despite normal sperm parameters.
