Influence of transcriptional factor Sp1 on expression of bile acids transporters MRP3 and MRP4
10.11659/jjssx.12E015210
- VernacularTitle:转录因子 Sp1对胆酸转运蛋白 MRP3、MRP4表达的影响
- Author:
Kun CHEN
;
Jin CHAI
;
Xinchan FENG
;
Ying CHENG
;
Liangjun ZHANG
;
Zhen HU
;
Lanfang CHEN
;
Wensheng CHEN
;
Yonghong HE
- Publication Type:Journal Article
- Keywords:
cholestasis;
transcription factor;
Sp1;
MRP3;
MRP4
- From:Journal of Regional Anatomy and Operative Surgery
2016;25(7):469-472,473
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of transcriptional factor Sp1 on expression of bile acids transporters MRP3 and MRP4 so as to perfect the regulatory mechanism of MRP3 and MRP4 expression.Methods Transformed Sp1-overexpression and Sp1 siRNA plas-mids to HepG2 cell and obtained the stably cells line.Then the expression levels of bile acids transporters MRP3 and MRP4 were measured by RT-qPCR,and the change of protein levels were detected by Western blot.Results The stably cells line Sp1-OE-HepG2 and Sp1siRNA-HepG2 were successfully transformed.The mRNA expression and protein levels of MRP3 and MRP4 were significantly increased in Sp1-OE-HepG2 cells,among which the mRNA expression of MRP3 mRNA increased 2.8 times,the protein levels of MRP3 increased 3.0 times,and the mRNA expression and protein levels of MRP4 increased 3.2 times and 2.5 times respectively.Conversely,the mRNA expression and protein levels of MRP3 and MRP4 were decreased in Sp1 siRNA-HepG2 cells,among which the mRNA expression of MRP3 mRNA de-creased 52%,the mRNA expression of MRP4 mRNA decreased 58%,the protein levels of MRP3 decreased 57%,and the protein levels of MRP4 decreased 60%.Conclusion Transcriptional factor Sp1 could regulate the expression of bile acids transporters MRP3 and MRP4 in HepG2 cells.
