Histochemical Electron Microscopic Findings of the Distribution of the Glycosaminoglycans of Cultured Sensory Retina.
- Author:
Jin Sang KIM
1
;
Ji Won KWON
;
Myung Kyoo KO
Author Information
1. Department of Ophthalmology, College of Medicine, Hanyang University Hospital, Korea. fovea@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Culture;
Electron microscopy;
Glycosaminoglycans;
Retina;
Ruthenium red
- MeSH:
Axons;
Culture Media;
Dendrites;
Extracellular Space;
Glycosaminoglycans*;
Humans;
Membranes;
Microscopy, Electron;
Microscopy, Electron, Transmission;
Retina*;
Retinal Neurons;
Retinaldehyde;
Ruthenium Red;
Tissue Donors
- From:Journal of the Korean Ophthalmological Society
2002;43(1):197-203
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study was performed to investigate the distribution of glycosaminiglycans by the activated retinal neuronal cell on the cultured retinal tissue. METHODS: The retinal tissue was obtained from the donor eyeball without the pathological findings of the retina. The dissected retinal tissue was cultured for 2 weeks in the culture media and reacted with ruthenium red dye to observe the glycosaminoglycans reaction with transmission electron microscopy. RESULTS: The histochemical reaction to ruthenium red on the cultured retina tissue was prominent on the internal limiting membrane, intercellular space between the axons and dendrites at the outer and inner plexiform layers and interphotoreceptor matrix. CONCLUSIONS: These findings suggest that GAGs may be released into the extracellular spaces between the axons and dendrites, which modulate the retinal circuits.
