Expression of GAP-43 in Midbrain Ventral Tegmental Area of Morphine With-drawal Rats

VernacularTitle:吗啡戒断大鼠中脑腹侧背盖区GAP-43的表达变化
Author: Liangming LUO ; Yufeng WU ; Weicheng NI ; Hua ZHU
Publication Type:Journal Article
Keywords: forensic pathology; morphine dependence; grow thassociated protein-43; rats
From: Journal of Forensic Medicine 2013;(5):330-332,336
CountryChina
Language:Chinese
Abstract: Objective To observe the protein expression of grow thassociated protein-43 (GAP-43) in mid-brain ventral tegmental area in morphine withdrawal rats at different time, and to evaluate the effect of GAP-43 on morphine withdrawal memory. Methods Rat models of morphine dependent 1 week, 2 weeks and 4 weeks were established by morphine hydrochloride intraperitoneal injection with increasing doses to establish natural withdrawal. The protein expression of GAP-43 in midbrain ventral tegmental area was observed by im munohistochemical staining and the results were analyzed by Im age-Pro Plus 5.1 im-age analysis system . Results With prolongation of dependent time, the expression of GAP-43 was de-creased then increased in midbrain ventral tegmental area . Conclusion GAP-43 could play arole in morphine withdrawal memory in midbrain ventral tegmental area.