Expression of MMP-2 and NF-κB in myocardium of mice with viral myo-carditis
10.3969/j.issn.1000-4718.2016.09.029
- VernacularTitle:病毒性心肌炎小鼠心肌中 MMP－2和 NF－κB 的表达
- Author:
Miaohua RUAN
;
Kai WANG
;
Dan WANG
;
Aihua ZHOU
;
Maoping CHU
;
Qi CHEN
;
Yan QIAN
- Publication Type:Journal Article
- Keywords:
Myocarditis;
Matrix metalloproteinase-2;
Nuclear factor-κB
- From:
Chinese Journal of Pathophysiology
2016;32(9):1704-1707,1712
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM: To observe the effects of TNF-α/nuclear factor-κB( NF-κB) /matrix metalloproteinase-2 (MMP-2) pathway on the expression of MMP-2 in the mice with viral myocarditis.METHODS: Six-week-old inbred male mice were randomly assigned to control and myocarditis group.The mice in myocarditis group and control group were intra-peritoneally inoculated with 0.1 mL 10-5.69 TCID50 /mL coxsackievirus B3 and vehicle (PBS), respectively.Ten mice were sacrificed at the 4th and 10th days after injection.The blood and heart specimens were harvested.The serum content of TNF-αwas measured by ELISA.The myocardial levels of MMP-2, NF-κB p65 and IκBαwere determined by Western blot.RESULTS: Compared with control group, the protein expression of MMP-2 and NF-κB p65 in the myocardium and the serum content of TNF-αwere significantly increased in myocarditis group (P <0.05).The protein expression of IκBαwas lower in myocarditis group than that in control group (P <0.05).CONCLUSION: TNF-α, NF-κB p65 and MMP-2 were higher in the mice with acute viral myocarditis.The increased expression of them might be involved in the pathogene-sis of viral myocarditis.
