A comparative study on the quantitative detection of two reagents of D-Dimer by automatic ;coagulation analyzer
10.3969/J.ISSN.1672-8270.2016.09.015
- VernacularTitle:全自动血凝仪定量检测D-二聚体两种试剂的比较研究
- Author:
Xiuqing ZHAO
;
Xiaohui WU
- Publication Type:Journal Article
- Keywords:
D-Dimer reagent;
Anti-interference;
Laboratory diagnosis;
Coagulation analyzer
- From:
China Medical Equipment
2016;13(9):49-51,52
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the detection ability of two reagents by automated coagulation analyzer ACL-TOP using immune turbidimetric method, and to determine the merits and demerits of them. Methods:Two reagents were compared in the study. We selected 532 cases of hospitalized patients in Anzhen Hospital, 388 cases of which were unstable angina, 60 cases were pulmonary thromboembolism, 70 cases were coronary atherosclerosis, 14 cases were abdominal aneurysm, and the rest were the patients with high blood pressure and coronary heart disease. The plasmas of all the patients were extracted, and D-dimer was detected on ACL-TOP by D-Dimer (referred to as reagent A) and D-DHS (referred to as reagent B). Results:The results of D-dimer increased in different degrees, and there are 88 cases of false positive cases. The case whose FDP and individual D-dimer of D-dimer simultaneous detection was elevating and clinical symptoms were disappeared was detected by the VIDAS instrument platform verification. There were significant differences between A and B. The detection result of reagent A was [1594.15±2286.18], and reagent B was [912.97±1814.06]. There were significant differences between them (t=3.654, P<0.05). In addition, the consistency of the two reagents was poor (r=0.419, P<0.05). Conclusion:Comparing two reagents to detect D-dimer quantitatively by automated coagulation analyzer ACL-TOP, D-DHS owns greater ability to exclude false-positive results, so does the ability of anti-interference with jaundice, hemolysis and chylemia.
