Diversity of SCCmec type diversity in clinically isolated methicillin-resistant Staphylococcus epidermidis
10.3969/j.issn.1673-4130.2016.16.026
- VernacularTitle:临床分离耐甲氧西林表皮葡萄球菌SCCmec型别多样性分析
- Author:
Yongchang YANG
;
Liang CHEN
;
Daiwen XIAO
;
Hua YU
;
Hua LIU
;
Wenfang HUANG
- Publication Type:Journal Article
- Keywords:
methicillin-resistant Staphylococcus epidermidis;
SCCmec type;
MRSE;
diversity
- From:
International Journal of Laboratory Medicine
2016;37(16):2273-2274,2277
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the SCCmec types of clinically isolated methicillin‐resistant Staphylococcus epidermidis (M RSE) .Methods Eighty‐four strains of clinically isolated Staphylococcus epidermidis identified by the fully automatic microbio‐logical identification system were collected and performed the MRSE identification by PCR for amplifying esp and mecA genes and SCCmec typing .Its distribution characteristics were analyzed .Results Esp gene was amplified in 84 strains and the detection rate of mecA was 76 .19% (64/84) ,in which the MRSE detection rates in blood ,sputum ,urine and wound secretion were 76 .8% , 68 .8% ,100% and 71 .4% respectively .The multiple PCR amplification displayed that among 64 strains of MRSE ,19 strains were SCCmec simple type ,in which 19 strains were SCCmec type Ⅰ and 3 strains were SCCmec type Ⅲ ;42 strains were SCCmec mixed type ,in which 2 strains were SCCmec mixed type Ⅰ and Ⅱ ,14 strains were SCCmec mixed type Ⅰ and Ⅲ ,12 strains were SCCmec mixed type Ⅰ ,Ⅱ and Ⅲ ,5 strains were SCCmec mixed type Ⅱ and Ⅲ ,a strains were and SCCmec mixed type Ⅲ and Ⅳ .Conclu‐sion The SCCmec type in clinically isolated MRSE shows obvious diversity and its majority is SCCmec mixed type .
