Application of real-time PCR in rapid detection of bloodstream infection pathogens
10.3969/j.issn.1673-4130.2016.16.028
- VernacularTitle:实时定量PC R在血流感染病原体快速检测中的应用
- Author:
Shizhen FAN
;
Songqing LIN
- Publication Type:Journal Article
- Keywords:
bloodstream infection;
sepsis;
real-time PCR
- From:
International Journal of Laboratory Medicine
2016;37(16):2278-2279,2282
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical application value of real‐time PCR in the detection of bloodstream infection pathogens .Methods A total of 92 blood samples from 80 patients in our hospital were collected for conducting real time PCR de‐tection and conventional blood culture .The sensitivity and specificity were compared between the two methods .Results Among 92 samples ,66 samples (71 .7% ) were negative in both assays .Ten different pathogens were detected by either blood culture system or real‐time PCR or by both methods .Seven positive samples were detected by both assays .The consistence of the two methods was 79 .3% .The negative predictive value of real‐time PCR was 0 .94 ,the sensitivity was 0 .64 and the specificity was 0 .82 .Among them ,15 samples were positive in real‐time PCR ,while negative in blood culture system ,4 samples were positive in the blood cul‐ture ,whereas were negative in the real‐time PCR .The pathogens cultured in 2 samples were not in the detection range of real time PCR ,moreover real time pCR could not detect Candida glabrata .Conclusion Real time PCR is a valuable method for rapidly detec‐tion the sample of bloodstream infection ,but cannot completely replace the blood culture test .
