Determination of methomyl in human blood by LC/MS
10.13618/j.issn.1001-5728.2016.04.011
- VernacularTitle:液相色谱-质谱联用法测定人全血中灭多威
- Author:
Meiling ZHANG
;
Zhennan ZHANG
;
Congcong WEN
;
Zhiyi WANG
;
Dan LIN
;
Xianqin WANG
- Publication Type:Journal Article
- Keywords:
forensic toxicological analysis;
LC/MS;
methomyl;
blood
- From:
Chinese Journal of Forensic Medicine
2016;31(4):367-368,372
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a sensitive and simple LC/MS method for determination of methomyl in blood samples.Methods Methomyl was extracted from blood by liquid-liquid extraction with ethyl acetate, and then analyzed on a Zorbax SB-C18 (2.1mm×50mm, 5μm) column. The mobile phase consisted of acetonitrile-0.1% formic acid with gradient elution, at a lfow rate of 0.5 mL/min, at 40℃. LC-MS was performed in ESI source with MRM mode for quantiifcation.ResultsThe linear range of the concentration were 0.05~2.0μg/mL for methomyl (r>0.995). The relative recoveries of methomyl were in the range of 90%~108%. The RSDs of intra-days and inter-day were both less than 15%.Conclusion The method is a simple, quick, sensitive and could be used for determination of methomyl in blood samples.
