Changes of miRNA-21 in the peripheral blood in patients with chronic heart failure and its clinical significance
10.3969/j.issn.1006-5725.2016.15.015
- VernacularTitle:慢性心力衰竭患者血miRNA-21的变化及意义
- Author:
Ping QIAO
;
Jianlin MA
;
Jing LIN
;
Lining MA
;
Qingliu WU
- Publication Type:Journal Article
- Keywords:
Chronic heart failure;
Real-time fluorescence quantitative PCR;
MiRNA-21
- From:
The Journal of Practical Medicine
2016;32(15):2471-2473,2474
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression difference of microRNA -21 (miRNA-21) in patients with chronic cardiac function and analyze its clinical significance. Methods Patients with chronic heart failure (trial group,150 cases) and the subjects without chronic heart failure (control group,45 cases) were enrolled. Patients with chronic heart failure were divided into three subgroups according to NYHA: group A (heart function classⅡ,49 cases),group B (classⅢ, 51 cases) and group C (class Ⅳ, 50 cases). miRNA-21 levels were detected by qRT-PCR method. The levels of B type natriuretic peptide urea (BNP),left ventricular end diastole diameter (LVEDD) and left ventricular ejection fraction (LVEF) were determined. Results miRNA-21 expression in patients with heart failure were higher than that of control group (P < 0.01),and miRNA-21 expression in group C was higher than that of group A.Correlation analysis showed that there was a positive correlation between expression of BNP (r = 0.763,P < 0.01), LVEDD (r = 0.691,P<0.01), and the level of miRNA-21 in patients with chronic heart failure.And a negative correlation between LVEF value and the level of miRNA-21 (r = -0.918,P < 0.01). Conclusion miRNA-21 might be a potential marker for diagnosis of heart failure and could be a basis for reference about prognosis evaluation.
