Chromokinesin KIF4A as a tumor suppressor by inhibiting cell invasion
10.3760/cma.j.issn.1673-4181.2016.03.003
- VernacularTitle:驱动蛋白KIF4A抑制胃癌细胞的侵袭作用
- Author:
Meichao JI
;
Hongyu JIANG
;
Zhixiong DONG
;
Changjun ZHU
- Publication Type:Journal Article
- Keywords:
KIF4A;
Cortactin;
Cell invasion;
Gastric cancer cell
- From:
International Journal of Biomedical Engineering
2016;39(3):140-143,前插5
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of chromokinesin KIF4A in gastric cancer cell invasion using gastric cancer cells SGC-7901 and chromokinesin KIF4A deficient gastric cancer cells (SGC-shKIF4A).Methods Expression levels of KIF4A in controlling gastric cancer cells (SGC-shNC) and SGC-shKIF4A cells were determined by Western Blot.Invasion of gastric cancer cells were assessed using Transwell invasion assay and the number of cells passing through the matrigel was counted.Changing numbers of cortactin in SGC-7901,SGC-shNC,and SGC-shKIF4A cells were analyzed by immunofluorescence staining.Results Compared to the SGC-shNC cells,invasion ability of SGC-shKIF4A cells was increased.Compared to other cells,the numbers of cortactin in SGC-shKlF4A cells was also increased suggesting invadopodia in these cells was increased(P＜0.01).Conclusions Chromokinesin KIF4A acts as a tumor suppressor by inhibiting gastric cancer cells invasion and the results provides strong evidences for KIF4A serving as one of potent targets for gastric cancer prognostics and treatment in clinic.
