The protective effect and mechanism of TLR2 on permeability of intestinal epithelial barrier
10.3760/cma.j.issn.1673-4408.2016.06.015
- VernacularTitle:Toll样受体2对肠上皮细胞屏障通透性的保护作用及机制
- Author:
Nan LIN
;
Lingfen XU
;
Xu TENG
;
Mei SUN
- Publication Type:Journal Article
- Keywords:
Intestinal epithelial barrier;
Transepithelial electrical resistance;
TLR2;
PI3K/Akt pathway;
Inflammatory bowel disease
- From:
International Journal of Pediatrics
2016;43(6):484-487,501
- CountryChina
- Language:Chinese
-
Abstract:
Objective Intestinal epithelial barrier damage is closely related to a variety of gastrointestinal disease,how to maintain its function effectively is the key to treat all these diseases.This research attempts to explore the protective effect and its mechanism of toll-like receptor 2 (Toll-like receptor,TLR2)on permeability of intestinal epithelial barrier by experiments in vitro,to lay a foundation for new treatment methods.Methods We cultured non-transfected Caco-2 cells,TLR2-deficiency Caco-2 cells,TLR2-overexpressed Caco-2 cells in normal control group until the 21 st d,then tested transepithelial electrical resistance(TEER) which reacts the permeability of epithelial barrier.We cultured 3 types of cells in inflammation group until the 19th d treated with 10 ng/ml IL-1 beta for 48 h,then tested TEER values at the 21st d.We treated 3 types of cells in inhibition group with PI3K/Akt pathway inhibitor for 1h befor IL-1 beta,then tested TEER values at the 21st d.Results TEER value of TLR2-deficiency Caco-2 cell monolayer significantly reduced (P ＜ 0.01),whereas TEER value of TLR2-overexpressed Caco-2 monolayer raised,but without statistically significant.TLR2 can prevent IL-1 beta caused TEER decreasing (P ＜ 0.01),but the effect disappeared after given PI3K/Akt pathway inhibitor.Conclusion TLR2 can regulate the permeability of intestinal epithelial barrier.In addition,TLR2 can protect permeability increasing caused by inflammation,this effect mediated by PI3 K/Akt pathway.
