Effect of flavopiridol on the proliferation, invasiveness and apoptosis of human prostatic cancer cell line LNCaP
10.3760/cma.j.issn.1006-9801.2016.06.002
- VernacularTitle:flavopiridol对前列腺癌细胞LNCaP增殖、侵袭及凋亡的影响
- Author:
Ning LI
;
Gang LIANG
;
Hong XIAO
;
Huixia ZHENG
;
Jianfang LIANG
- Publication Type:Journal Article
- Keywords:
Prostatic neoplasms;
Flavopiridol;
Proliferation;
Apoptosis;
Invasiveness
- From:
Cancer Research and Clinic
2016;28(6):366-368,372
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of flavopiridol on the proliferation,invasiveness and apoptosis of human prostate cancer cell line LNCaP,and to explore the possibility of its application in clinical treatment.Methods MTT assay was used to detect cell proliferation,cell invasion in vitro was detected by Transwell assay,and flow cytometer was used to observe apoptosis.Results Flavopiridol inhibited the growth of LNCaP cells in a concentration-dependent and time-dependent way (P ＜ 0.05),and reduced the ability of invasion capacity.After treated by 10 nmol/L flavopiridol for 24 h,the apoptosis rate was increased significantly to (7.5±0.9) ％ compared with the control group [(5.3±0.5) ％] (P ＜ 0.05).Conclusion Flavopiridol can inhibit proliferation of LNCaP cells and induce apoptosis,which may be applicable for the treatment of prostate cancer.
