Effect of PLTP on CSE-induced IL-8 production in human bronchial epithelial cells
10.3969/j.issn.1000-484X.2016.07.004
- VernacularTitle:PLTP在香烟诱导HBECs合成IL-8中的作用
- Author:
Xiuying YU
;
Youlun LI
;
Fengping WU
;
Yajuan CHEN
- Publication Type:Journal Article
- Keywords:
Cigarette smoke extract;
Human bronchial epithelial cells;
HBECs;
PLTP;
IL-8
- From:
Chinese Journal of Immunology
2016;32(7):952-956,961
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of PLTP gene on CSE-induced IL-8 production in human bronchial epithelial cell line (HBECs). Methods:Wistar rats were exposed to air or cigarette smoke for 6 hours/day on 3 consecutive days,then the lungs were sectioned and examined. The number of total white blood cell and differential white blood cells in BALF were counted. The different concentrations of CSE co-cultured with HBECs for 24 hours. Cells growth was detected by MTT assay. Expression levels of PLTP mRNA and IL-8 mRNA were examined by RT-PCR,protein of PLTP was investigated by Western blot,and production of IL-8 ex-amined by ELISA. Results:The number of white blood cells in BALF was significantly increased compared with controls. Enhanced ex-pression level of PLTP and IL-8 were observed in CS-exposure group. Proliferation of HBECs tends to decrease at high concentrations of CSE(2. 0% CSE and 4. 0% CSE). The results suggested that the production of IL-8 induced by CSE in a time- and concentration-dependent manner,while the expression of PLTP induced by CSE in a dose-dependent manner. Furthermore,expression levels of IL-8 significantly increased after silence PLTP gene. Conclusion:PLTP siRNA could increase CSE-induced IL-8 production in HBECs.
