Preliminary research in relationship between TNFAIP8 and gastric cancer drug resistance

VernacularTitle:TNFAIP8与胃癌耐药关系的初步研究
Author: Lina LI ; Zhibin BI ; Jinsheng WANG
Publication Type:Journal Article
Keywords: Gastric carcinoma; TNFAIP8; Drug resistance
From: Chinese Journal of Immunology 2016;32(7):962-964,969
CountryChina
Language:Chinese
Abstract: Objective:To explore if there was a correlation between TNFAIP8 and gastric tumor drug resistance. Methods:We collected 47 cases of neoadjuvant chemotherapy,and detected the expression of TNFAIP8 in their tissues before and after surgery by im-munohistochemistry. Compared the expression of TNFAIP8 between SGC7901 cell and its cisplatin resistance cell SGC7901-DDP by Western blot,and then we used siRNA technology to decreased its expression,continued to test the susceptibility to cisplatin through MTT. Results:The expression of TNFAIP8 between pre-neoadjuvant and after-neoadjuvant had no difference, but the expression of chemotherapy effectiveness was lower than chemotherapy invalidness(P<0. 05);the expression of SGC7901-DDP cell was also higher than SGC7901 cell; the expression of TNFAIP8 decreased apparently through plasmid transfection of SGC7901-DDP cell, MTT test showed the fewer expression of TNFAIP8 could increase the susceptibility to cisplatin(P<0. 05). Conclusion: TNFAIP8 was involved in chemotherapy resistance of gastric carcinoma.