Role of NADPH oxidase in high fat-induced injury in MIN6 islet βcells
10.3969/j.issn.1001-1978.2016.09.025
- VernacularTitle:NADPH氧化酶在高脂诱导的MIN6胰岛β细胞损伤中的作用
- Author:
Jing LI
;
Dan CHEN
;
Yu ZHOU
;
Cuiyu BAO
- Publication Type:Journal Article
- Keywords:
NADPH oxidase;
high fat;
MIN6;
islet βcells;
Bcl-2;
Bax
- From:
Chinese Pharmacological Bulletin
2016;32(9):1317-1320,1321
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the role of nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase in high fat-induced injury in MIN6 isletβ cells. Methods MIN6 islet β cells were exposed to different concen-trations of palmitic acid ( 0. 1 , 0. 3 , 0. 5 , 0. 8 mmol · L-1 ) for 48 h and different time points of 0. 5 mmol· L-1 palmitic acid(24,48,72,96 h). Cell viability was measured by MTT, the protein expression of NADPH oxidase subunits such as p22 phox , p47 phox , p67 phox and gp91 phox and apoptosis related proteins such as Bcl-2 and Bax were determined by Western blot. Results MIN6 islet cells exposed to palmitic acid at 0. 5 mmol ·L-1 for 48 h showed a decrease in their viability and an increase in the expression of NADPH oxidase sub-units(p22phox、p47phox、p67phox and gp91phox) and Bax(P<0. 05 ) , while Bcl-2 expression was significantly re-duced. the pretreat with NADPH oxidase inhibitor di-phenyliodonium( DPI,10 μmol · L-1 ) significantly in-hibited high fat-induced Bcl-2 and Bax expression( P<0. 05 ) . Conclusion Activated NADPH oxidase might play an important role in the treatment of high fat-in-duced injury in MIN6 islet cells.
