Effect of gypenosides on RAGE and TGF-β1 expression in human mesangial cells induced by AGEs
10.3969/j.issn.1001-1978.2016.09.022
- VernacularTitle:绞股蓝皂苷对AGEs诱导下人肾小球系膜细胞中RAGE及转化生长因子-β1表达的影响
- Author:
Qiuyan ZHANG
;
Ling TANG
;
Yan WANG
;
Kang ZHOU
;
Huiyun ZHANG
- Publication Type:Journal Article
- Keywords:
gypenosides;
diabetic nephropathy;
human mesangial cells;
advanced glycation endproducts;
recep-tor for advanced glycated endproducts;
TGF-β1;
fibrosis
- From:
Chinese Pharmacological Bulletin
2016;32(9):1301-1305,1306
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the effect of gypenosides ( GP) on the expression of receptor for advanced gly-cated endproducts ( RAGE ) and transforming growth factor-β1 ( TGF-β1 ) in human mesangial cells( HMCs) induced by AGEs. Methods HMCs were cultured in DMEM of low glucose containing 15% fetal bovine ser-um in vitro, which were divided into four groups: the normal group, model group, GP group and positive control group. In addition to the normal group, the other groups were stimulated by AGEs ( 200 mg · L-1 );furthermore, GP group was intervened with dif-ferent concentrations(25,75,175 mg·L-1) of GP, while control group was given 10 mmol · L-1 of amin-oguanidine hydrochloride. The expression of RAGE and TGF-β1 protein of each group was detected by Western blot; the expression of RAGE and TGF-β1 mRNA of each group was detected by RT-PCR. Re-sults The expression of RAGE, TGF-β1 protein and mRNA in HMCs induced by AGEs in the model group was significantly higher than that of the normal group ( P<0. 01 );compared with the positive control group ( P<0. 01 ) , GP could obviously reduce the expression of RAGE, TGF-β1 protein and mRNA in a dose-de-pendent manner. Conclusion GP can reduce the ex-pression of RAGE in HMCs induced by AGEs, block AGEs-RAGE signaling pathway and decrease the ex-pression of the downstream factor TGF-β1 , therefore, it plays the role in the resistance of rennal fibrosis in DN.
