Resveratrol inhibited hepatic fibrosis in mice with schistosomiasis japonica by modulating Th1 and Th2 responses
10.3969/j.issn.1001-1978.2016.08.012
- VernacularTitle:白藜芦醇调控 Th1和 Th2应答抑制小鼠日本血吸虫病肝脏纤维化的研究
- Author:
Weiwei ZHANG
;
Jifeng ZHU
;
Ren WANG
;
Yanan GAO
;
Junfeng ZHANG
;
Shujuan TONG
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
resveratrol;
praz-iquantel;
fibrosis;
Th1;
Th2
- From:
Chinese Pharmacological Bulletin
2016;32(8):1091-1096,1097
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the antifibrogenic effect of resveratrol on mice with schistosomiasis japonica and its effect on Th1 and Th2 responses .Methods Forty-five mice infected with S.japonicum cercariae for 3 weeks were randomly divided into three groups named as infection group ( A) , resveratrol group ( B) and praz-iquantel group ( C) .Fifteen normal mice were taken as normal control group ( D) .In the 13th week post-infec-tion, all mice were sacrificed and the liver tissues were removed.Histopathological changes were observed in the liver of all groups .Splenocytes were prepared from spleens of mice with S.japonicum infection and the proportions of Th1 and Th2 cells in T cells were deter-mined by FACS respectively .RT-PCR was used to de-tect the relative IFN-γ,IL-13,TGF-βmRNA levels in liver tissue .Results After treatment , the degrees of liver fibrosis in groups B and C decreased in the 13th week post-infection ( P <0.01 ) .Compared to group A, the proportions of Th1 cells in group B significantly increased ( P<0.05 ) and the proportions of Th 2 cells in group B decreased significantly ( P <0.01 ) .The level of anti-SWA IgG 2 a in group B was significantly higher ( P<0.05) , while the anti-SEA IgG1 level in group B was lower ( P <0.01 ) than that in group A . The hepatic expression of IFN-γmRNA level in group B was higher than that in group A ( P<0.05 ) , and IL-13 ,TGF-βmRNA levels in group B were lower than in group A ( P<0.01 ) .Conclusion Resveratrol has an antifibrogenic effect through upregulating Th 1 cell re-sponse and downregulating Th 2 cell response in mice infected with Schistosoma japonicum.
