Effect of miRNA-143 on homocysteine induced vascular smooth muscle cell proliferation
10.3969/j.issn.1001-1978.2016.08.013
- VernacularTitle:miRNA－143在同型半胱氨酸致血管平滑肌细胞增殖中作用的研究
- Author:
Xiaoling YANG
;
Fengying GUO
;
Shengchao MA
;
Anning YANG
;
Chengjian CAO
;
Yuexia JIA
;
Guizhong LI
;
Yideng JIANG
- Publication Type:Journal Article
- Keywords:
miRNA-143;
homocysteine;
vascular smooth muscle cells;
proliferation;
methylation;
ex-pression
- From:
Chinese Pharmacological Bulletin
2016;32(8):1097-1100,1101
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of miRNA-143 ( miR-1 4 3 ) on homocysteine ( Hcy ) induced-vascular smooth muscle cells ( VSMCs ) proliferation and the mechanism .Methods VSMCs were cultured and in-cubated with Hcy by using primary cultured method . Then, cells were treated with different concentrations of Hcy and folate .VSMCs proliferation was determined with MTT assay , miR-143 was measured by qRT-PCR, and methylation of miR-143 was determined with meth-ylated PCR.Results After cells were treated with dif-ferent concentrations of Hcy , the proliferation of VSMCs was significantly increased , mRNA expression of miR-143 was decreased and methylation of miR-143 was increased .The proliferation of VSMCs was signifi-cantly decreased when transfected VSMCs with miR-143 precursor , and cell proliferation was increased by using miR-143 inhibitor transfection .Conclusion Hy-pomethylation of miR-143 may inhibit VSMCs prolifera-tion.
