Study of PIK3CA gene mutation status in different clinicopathological characteristics of breast cancer
10.3969/j.issn.1673-4130.2016.15.022
- VernacularTitle:PIK3CA 基因突变状态在乳腺癌不同临床病理特征中的研究
- Author:
Yuemin DENG
;
Yunjian XU
- Publication Type:Journal Article
- Keywords:
breast cancer;
PIK3CA;
mutation
- From:
International Journal of Laboratory Medicine
2016;37(15):2110-2111,2114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the mutation status of PIK3CA genes in breast cancer and to analyze its relation with clinico‐pathological characteristics .Methods The paraffin tissue samples in 176 cases of breast cancer were selected and the tissue DNA was extracted ,exon9 and exon20 were amplified by ARMS‐PCR .The mutation status of PIK3CA genes were detected ,other tissue samples in 20 cases of mammary gland disease were taken as the negative control .Results Among 176 samples of breast cancer tis‐sues ,45 cases of mutation were found with the mutation rate of 25 .6% ,which was dominated by H1047R mutation rate of exon20 with the mutation rate of 26 .7% (12/45);There were statistical difference between ER (+ ) and ER(-) and between PR(+ ) and PR(-) ,but no statistical difference was found between HER (+ ) and HER(-);PIK3CA gene mutation had correlation with cancer histological grade ,but no obvious correlation with tumor size ,age and lymph node status .Conclusion PIK3CA gene muta‐tion is correlated with breast cancer hormone receptor (HR) expression and tumor progression ;the PIK3CA gene mutation detection has an important significance for guiding the formulation of clinical indiidualized treatment .
