Protective effects of NDP on LPS induced neuron injuries in rat mixed cultures
10.3969/j.issn.1001-1978.2016.08.022
- VernacularTitle:金钗石斛多糖对脂多糖作用大鼠皮层胶质细胞－神经元体系的保护作用
- Author:
Mu LIN
;
Qihai GONG
;
Qin WU
;
Feng ZHANG
;
Jingshan SHI
- Publication Type:Journal Article
- Keywords:
noble dendrobium polysaccharides;
neu-rons;
microglia;
lipopolysaccharide;
astrocytes;
in-flammatory factor
- From:
Chinese Pharmacological Bulletin
2016;32(8):1144-1147,1148
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effect of noble dendrobium polysaccharides ( NDP ) on lipopo-lysaccharide ( LPS)-induced neuron injuries in newborn rat cerebral cortex glial cells and neuron mixed cul-tures.Methods The primary cultures of newborn rat cortical neurons and glial cells were established and the existence of the neurons , astrocytes and microglia was verified respectively .NDP was given to LPS-induced mixed cultures , the mRNA levels of IL-1β, TNF-αand COX-2 were assayed by real time PCR .Results NDP reduced the glial cell activation and neuron dam-age after it was given to LPS-induced mixed cultures . The mRNA levels of IL-1β, TNF-α, COX-2 were re-duced .Conclusion NDP protects against LPS-in-duced neuron-inflammation in neurons and glial cells cultures.
