Study on expression of RACK1,Src and Bcl-2 protein and their correlation in gastric carcinoma tissues and adjacent carcinomatous tissues
10.3969/j.issn.1671-8348.2016.19.017
- VernacularTitle:胃癌与癌旁组织中RACK1、Src和Bcl-2蛋白的表达及相关性研究
- Author:
Junmao CHEN
;
Siyang LIU
;
Jinghua WU
;
Jianli CHEN
;
Peng ZHAO
;
Xiaotao WANG
;
Guanghua YANG
;
Changyou WANG
;
Guozhi ZHANG
;
Zhenxing LI
- Publication Type:Journal Article
- Keywords:
gastric carcinoma;
receptor for activated C kinase-1;
steroid receptor auxiliary activator;
B-cell lymphoma-2 gene
- From:
Chongqing Medicine
2016;45(19):2645-2647,2651
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression change of RACK1 ,Src and Bcl‐2 in gastric carcinoma tissue and adjacent carcinomatous tissue .Methods Eighty specimens of gastric carcinoma and adjacent carcinomatous tissues in our hospital from Au‐gust 1 ,2011 to February 1 ,2014 were collected .The immunohistochemistry staining and Western blotting methods were adopted to detect the expression of RACK1 ,Src and Bcl‐2 protein in gastric carcinoma and adjacent carcinomatous tissues ,and their correlation was analyzed and performed the statistical analysis by combining with the clinicopathological data .Results The immunohistochem‐istry staining and Western blotting detection displayed that the expression positive rate and expression level of RACK 1 in gastric carcinoma tissue were obviously lower than those in the adjacent carcinomatous tissue ,while the expression positive rate and ex‐pression level of Src and Bcl‐2 in gastric carcinoma tissue were obviously higher than those in the adjacent carcinomatous tissue ,the differences were statistically significant (P<0 .05) .The RACK1 expression in gastric carcinoma tissue was negatively correlated with the Src and Bcl‐2 expression(r= -0 .632 ,-0 .754 ,P<0 .01) ,while Src had no obvious correlation with Bcl‐2 protein(r=0 .217 ,P>0 .05) .Conclusion The expression of RACK1 in gastric carcinoma tissue is significantly decreased ,while the expres‐sions of Src and Bcl‐2 are increased .
