Heterotopic osteogenesis in vivo of PHBHHx and human umbilical cord mesenchymal stem cells
10.3969/j.issn.1671-8348.2016.20.002
- VernacularTitle:PHBHHx与人脐带间充质干细胞的体内异位成骨研究
- Author:
Zhixu HE
;
Mei WANG
;
Chuan YE
;
Yong WANG
;
Xiao ZENG
;
Junbiao ZHANG
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
tissue engineering;
bone;
PHBHHx
- From:
Chongqing Medicine
2016;45(20):2740-2743,2747
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the ability for constructing tissue engineering bone in vivo in complex scaffolds with PHB‐HHx as the scaffolds material and human umbilical cord mensenchymal stem cells (hUCMCs) as the seed cells .Methods hUCM‐SCs were inoculated into PHBHHx scaffolds to induce osteogenesis culture in vivo for two weeks ,the the induced group was the experimental group and those without instilling hUCMCs served as the control group ,the nude mouse subcutaneous implantation was performed .Then taking material at 1 ,3 ,5 months after implantation in vivo was performed for conducting HE ,collagenⅠim‐munohistochemical ,alkaline phosphatase staining and RT‐PCR .Results hUCMSCs showed good cellular adsorbability .The size and form in the experimental group basically maintained the original status ,and the osteogenesis specific indicators were positive ;but the control group did not keek the original status ,its volume was gradually shrunk until complete degradation ,and the osteogen‐esis specific indicators were negative .Conclusion The PHBHHx scaffolds combined with hUCMSCs has the capability of in vivo heterotopic constructing tissue engineering bone in nude mouse by in vitro osteogenic induction .
