Performance evaluation and comparison of different chemiluminescence immunoassay systems in detection of cortisol
10.3969/j.issn.1673-4130.2016.14.019
- VernacularTitle:不同化学发光分析系统在皮质醇检测中的性能评价与比较
- Author:
Huanhui CHEN
;
Tao LIN
- Publication Type:Journal Article
- Keywords:
cortisol;
chemoluminescence;
electrochemical luminescence;
performance evaluation
- From:
International Journal of Laboratory Medicine
2016;37(14):1949-1950,1953
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the analytical performance and results correlation of cortisol detection by the Beckman Access 2 Immunoassay System chemiluminescence system and the Roche COBAS E 411 electrochemical luminescence system .Methods The precision ,functional sensitivity ,reference range ,positive coincidence rate and negative coincidence rate of both Beckman Access 2 Immunoassay System and Roche COBAS E 411 systems for detecting cortisol were evaluated according to the method of ISO15198 .Results Both Beckman Access 2 Immunoassay System and Roche COBAS E 411 system exhibited better precision ,the functional sensitivity conformed to the lower limit of clinical detection set by the manufacturers ,after establishing the reference range of cortisol by the laboratory ,the cortisol values detected by these two systems were performed the linear regression analysis , the results indicated that the correlation between them was good ,the linear regression coefficient r2 =0 .977 ,and the positive and negative coincidence rates were 100% .Conclusion The two analytical systems have excellent performance for cortisol ,which all meet the clinical detection requirements .The methodological comparison shows good correlation between them ,and the detection re‐sults could be cited between these two systems by the regression equation .
