Analysis on aleukocytic suspending red blood cell supernatant fragment and particle image changes at different stored periods by microscopic phase contrast technology
10.3969/j.issn.1673-4130.2016.14.015
- VernacularTitle:显微相衬技术分析去白细胞悬浮红细胞上清液不同存储期内细胞碎片及微粒的图像变化
- Author:
Zhihao DENG
;
Yamei GUAN
;
Weijian WU
;
Weiwei TUO
;
Yaoxiong HUANG
- Publication Type:Journal Article
- Keywords:
aleukocytic suspending red blood cell;
different stored periods;
microscopic phase contrast image
- From:
International Journal of Laboratory Medicine
2016;37(14):1939-1940,1943
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the cellular fragments and particle image changes of inventory aleucocytic suspending RBC produced by the storage time extension ,RBC damage or hemolysis in order to provide the revelatory experimental basis for the transfusion safety .Methods The supernatant was prepared from different stored days (3 ,7 ,14 ,21 d) of stock aleukocytic suspen‐ding RBC .The particles in supernatant were observed and morphologically analyzed by using the microscopic static image analytic technology .Results There were a small amounts of visible particles in the sample supernatant preserved for 3 ,7 d and the parti‐cles′sizes are similar to cells′;the number of particles began to significantly increase from 14 d and the diameter became smaller . The particles filled the entire field until 21 d ,showing fragmentary status .Conclusion The cellular fragments and particles in the supernatants of stock aleukocytic suspending RBC with the storage period exceeding 14 d are significantly increased and have signif‐icant difference compared with those stored for less than 14 d .These exogenous fragments and particles may become antigens and induce the body immune response ,lead to transfusion adverse reactions .It is recommended that the patients should be transfused with stock aleukocytic suspending RBC within a storage period of 14 d .
