Effect of Biyanqing Granule on Proliferation and Radiosensitivity of Nasopharyngeal Carcinoma CNE-2 Cells in vitro
- VernacularTitle:鼻咽清颗粒抑制鼻咽癌 CNE-2细胞体外增殖及对放疗敏感性的影响
- Author:
Caixia FAN
;
Zhixi CHEN
;
Shiping FAN
;
Shuzhen LAI
;
Jinkun ZHENG
;
Xinhua LAI
- Publication Type:Journal Article
- Keywords:
Biyanqing granule;
Nasopharyngeal carcinoma CNE-2 cells;
Cell proliferation;
Radiosensitivity
- From:
China Pharmacist
2016;19(7):1248-1251,1252
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the proliferation inhibition and radiosensitization of Biyanqing granule on nasopharyngeal carci -noma cell line CNE-2 in vitro.Methods:CNE-2 cells were cultured in vitro.The inhibition of Biyanqing granule on the proliferation of CNE-2 cell was evaluated by MTT assay .Radiosensitization was explored by clone formation assay , and cell cycle and apopotosis were observed by flow cytometry ( FCM) .Results:Biyanqing granule could inhibit the proliferation of CNE-2 in a time-and dose-dependent manner.The IC50 in 24, 48 and 72 h was 70.79, 60.13 and 51.63 mg· ml-1(calculated according to the weight of all medicinal ma-terial), respectively.The colony formation assay showed that Biyanqing granule combined with radiation could significantly reduce the colony formation of CNE-2 cells.With the concentration increase of the main drug , the colony formation of CNE-2 cells was reduced . The number of colony formation in the negative control group , the radiation group , 10 mg· ml-1 and 20 mg· ml-1 Biyanqing combined with radiation groups (calculated according to the weight of all medicinal material ) was significant different (P<0.05).With the main drug concentration increasing , the percentage of G 2/M phase and apoptotic cells were both increased , and compared with the con-trol group, the difference was significant (P<0.05).Conclusion:Biyanqing granule can not only inhibit CNE-2 cells but also block CNE-2 cells in G2/M to improve the radiosensitization of CNE-2 cells.
