Mechanisms of imatinib mesylate induced apoptosis of primary T cells
10.3969/j.issn.1000-484X.2016.09.005
- VernacularTitle:伊马替尼诱导原代T细胞凋亡作用机制研究
- Author:
Xiaohua CHEN
;
Huayun QIU
;
Shanshan SHI
;
Sha WU
;
Chen LIN
;
Yangqiu LI
- Publication Type:Journal Article
- Keywords:
Imatinib mesylate;
Primary CD3+T cells;
A20;
NF-κB
- From:
Chinese Journal of Immunology
2016;32(9):1268-1271,1275
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate mechanism of imatinib mesylate induced apoptosis of primary CD3+T cells.Methods:The CD3+T cells were stimulated by 0-100 nmol/L imatinib for 24 h,cell apoptosis was detected by flow cytometry;Caspase-3,Caspase-8, A20 and NF-κB expression levels were detected by Real-time quantitative PCR and Western blot.Results: IM significantly increased apoptosis of T cell;Caspase-3 and A20 gene expression levels were upregulated and NF-κB expression level was downregulated both in gene and protein levels.Conclusion:IM increased apoptosis of T cell by upregulating A20 expression.
