In vitro culture and migration assay of mouse smooth muscle progenitor cells
10.3969/j.issn.1001-1978.2016.07.007
- VernacularTitle:小鼠平滑肌祖细胞培养及其体内外迁移功能的研究
- Author:
Qing WANG
;
Hequan LI
;
Jianying ZHOU
- Publication Type:Journal Article
- Keywords:
smooth muscle progenitor cells;
mesen-chymal stem cells;
culture;
migration;
α-SMA;
PDGF-BB
- From:
Chinese Pharmacological Bulletin
2016;32(7):915-919,920
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish a reliable method for the culture of mouse smooth muscle progenitor cells and in-vestigate their migration ability .Methods Mesenchy-mal stem cells from compact bones were obtained from C57 BL/6 mice and stimulated with PDGF-BB to in-duce these cells to differentiate into smooth muscle pro-genitor cells. Morphological analysis , immunocyto-chemical and flow cytometric analysis were used to i-dentify the cell type and the migration ability was in-vestigated by the transwell system and flow cytometry . Result After PDGF-BB stimulation for 7 days, the cells showed spindle shape and started to express α-SMA as demonstrated by immunocytochemistry .After 21 days induction , Flow cytometric analysis revealed that over 70%of the cells expressed both CD 34 andα-SMA and 58.5%of the cells expressed SM-MHC.Mi-gration assay showed that the smooth muscle progenitor cells from culture could migrate in vivo and in vitro. Conclusions The culture of smooth muscle progenitor cells from compact bone-derived mesenchymal stem cells is easily operated with high yield rate and shorten culture period . Obtained smooth muscle progenitor cells from culture could migrate in vivo and in vitro, which is suitable for the mechanism studies .
