Inhibitory effect of silymarin on human osteosarcoma Saos-2 cells and its mechanism
10.3969/j.issn.1001-1978.2016.07.016
- VernacularTitle:水飞蓟素对人骨肉瘤 Saos-2细胞的抑制作用及相关机制
- Author:
Chen YAN
;
Yahua WU
;
Ziyao WANG
;
Changchang YIN
;
Min YIN
- Publication Type:Journal Article
- Keywords:
silymarin;
osteosarcoma;
proliferation;
apoptosis;
mechanism;
ERK signaling pathway;
caspase-3
- From:
Chinese Pharmacological Bulletin
2016;32(7):966-969,970
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of silymarin on proliferation and apoptosis of human osteosarcoma saos-2 cells and to explore its possible mechanisms .Meth-ods Control group and different concentration groups of silymarin were set , and Saos-2 cells were treated with silymarin .Celluar morphologic changes were ob-served by inverted phase contrast microscope .Prolifer-ation of cells was tested CCK-8 assay .Apoptosis rate of cells was analyzed by flow cytometry . ERK1/2、p-ERK1/2 and cleaved caspase-3 protein expression were measured by Western blot .Results Silymarin inhibi-ted the proliferation of Saos-2 cells in a time-dependent and concentration-dependent manner . Silymarin in-duced the apoptosis of Saos-2 cells in a concentration-dependent manner . Silymarin decreased p-ERK1/2 protein expression and increased cleaved caspase-3 protein expression in a concentration-dependent man-ner, while ERK1/2 protein expression had no obvious change.Conculsions Silymarin can inhibit the prolif-eration of human osteosarcoma saos-2 cells and induce apoptosis of them , and the mechanism may be related to inhibition of ERK signaling pathway and upregulated caspase-3 protein expression .
