Study on Enantiomer Separation for Atropine by Capillary Electrophoresis
- VernacularTitle:阿托品光学异构体的毛细管电泳法拆分研究
- Author:
Xiongfei WANG
;
Yikun SUN
;
Yuezhu DING
;
Shanshan QIAO
;
Ruijuan YUAN
- Publication Type:Journal Article
- Keywords:
Atropine;
Chiral separation;
Capillary electrophoresis
- From:
China Pharmacist
2016;19(7):1293-1295,1304
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the enantiomer separation for atropine by capillary electrophoresis .Methods:Capillary elec-trophoresis was used with an elastic quartz capillary column (60 cm ×75 mm, effective length of 40 cm).The concentration of phos-phate buffer was 30 mmol· L-1 .The high and time of injection was 10 cm and 5 s, respectively.The detection wavelength was 225 nm.The best separation conditions were selected including the type and concentration of chiral resolving agent , pH of the buffer solu-tion, operating voltage and organic solvent.Results:The optimum conditions of separation were as follows:the pH of buffer solution was 7.0, the concentration of S-β-CDP was 10 mg· ml-1 , and the operating voltage was 12 kV.Conclusion: The method is simple and fast, which can be used to se parate the optical isomers of atrpo ine.
