Effects of Huang-Lian-Jie-Du-Decotion containing serum on expressions of autophagy related gene in macrophages

VernacularTitle:黄连解毒汤含药血清对巨噬细胞自噬相关基因表达的影响
Author: Honghong YU ; Mali WU ; Zhiwei ZHANG ; Rui CHEN ; Yun WU ; Zhixuan DUAN ; Weiyi TIAN
Publication Type:Journal Article
Keywords: Huang-Lian-Jie-Du-Decotion; Autophagy; RAM264.7 macrophages; Atherosclerosis; Beclin1; mTOR
From: Chinese Journal of Immunology 2016;32(8):1150-1152,1164
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effects of Huang-Lian-Jie-Du-Decotion containing serum for the autophagy of RAM264.7 macrophages ,and to explore its possible anti-atherosclerosis mechanism .Methods:The SD rats were intervened with large , middle and small dose Huang-Lian-Jie-Du-Decotion to prepare containing drug serum .Cell proliferation was tested by MTT assay .The gene and protein expressions of Beclin 1, mTOR was assessed by fluorescent quantitative PCR and Western blot method .Results:Compared with the normal serum group ,Huang-Lian-Jie-Du-Decotion containing serum enhanced expression of the mRNA and protein level of Beclin1,and decreased the expression of mTOR and p-mTOR(P<0.05 or P<0.01).Conclusion:Huang-Lian-Jie-Du-Decotion containing serum could regulate the expressions of Beclin 1 and mTOR.These might be one of the mechanisms for preventing atheroscle-rosis.