Expression and diagnostic value of miRNA-15 a in prostate cancer
10.3969/j.issn.1000-484X.2016.08.030
- VernacularTitle:miRNA－15a在前列腺癌中的表达及诊断意义
- Author:
Chunhong YAO
;
Xinchu YUAN
;
Chong LIU
;
Jianping DENG
- Publication Type:Journal Article
- Keywords:
Prostate cancer;
Benign prostatic hyperplasia;
miRNA-15a
- From:
Chinese Journal of Immunology
2016;32(8):1219-1221,1224
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the diagnostic value of miRNA-15a in patients with prostate cancer.Methods:From January 2014 to January 2015,Gene expression of MiRNA-15a were examined in 36 serum and tumor tissue samples from prostate cancer ,40 benign prostatic hyperplasia ( BPH) and 40 health volunteers ,which examined by quantitative Real-time reverse transcription polymerase chain reaction(RT-qPCR).Results: The serum expression of miRNA-15a were (0.193±0.081),(0.359±0.04) and (0.376± 0.037 ) in patients with prostate cancer ,benign prostatic hyperplasia and healthy controls respectively .miRNA-15 a expression was sig-nificantly lower in BPH and healthy controls than patients with prostate cancer (P<0.05).There were no significant difference between BPH and control group(P>0.05).Serum miRNA-15a expression in patients with prostate cancer at different expression levels PSA , Gleason score,clinical stage,the difference between the presence of distant metastasis was statistically significant (P<0.05),and other pathological factors unrelated (P>0.05);Prostate cancer tumor tissue expression and PSA expression levels of miRNA-15a,Gleason score,clinical stage,presence of distant metastasis was statistically significant difference between (P<0.05),and other pathological factors unrelated(P>0.05).Conclusion:Low expression of miRNA-15a may be contributed in the development of prostate cancer ,and the expression levels miRNA-15a may be considered potential novel biomarkers for the diagnosis of prostate cancer .
