Determination of Anti-FIIαActivity in Compound Heparin Sodium Cream by a Trace Chromogenic Sub-strate Method
- VernacularTitle:微量生色底物法测定复方肝素钠乳膏抗Ⅱα因子活性
- Author:
Yanfang LIU
;
Hongda MA
;
Ming YAN
;
Mingxiao HOU
- Publication Type:Journal Article
- Keywords:
Trace chromogenic substrate method;
Compound heparin sodium cream;
Anti-FIIαactivity
- From:
China Pharmacist
2016;19(6):1071-1072,1073
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a trace chromogenic substrate method for the determination of anti-FIIαactivity in compound heparin sodium cream .Methods: The anti-FIIαactivity in compound heparin sodium cream was determined by a trace chromogenic substrate method according to the completely random design of experiment based on the amount reaction principle of 4*4 parallel lines in the biological test statistics method .Results:The calibration curve was linear within the range of 0.005 04 IU· ml-1-0.021 IU· ml-1(r=0.992).The average recovery was 101.6% with RSD of 2.76% (n=9).Conclusion: The method is accurate, reliable and reproducible , and can be used for evaluating the quality of compound heparin sodium cream .
