Comparative research on nucleoside drug resistance mutation and genotypes of chronic hepatitis B virus in two areas by PCR-LDR method
10.3969/j.issn.1673-4130.2016.13.026
- VernacularTitle:应用 PCR-LDR法比较2个地区慢性乙型肝炎病毒核苷类药物耐药突变和基因型
- Author:
Binbin DING
;
Zhengjun DUAN
- Publication Type:Journal Article
- Keywords:
PCR-LDR;
HBV;
drug resistance;
genotype
- From:
International Journal of Laboratory Medicine
2016;37(13):1814-1816,1819
- CountryChina
- Language:Chinese
-
Abstract:
Objective To perform a comparative research on nucleoside drug resistance mutation and genotypes of chronic hepa‐tits B virus in Shanghai and Lanzhou .Methods The serum samples(Shanghai ,194 cases) and (Lanzhou ,325 cases) were collected . The common 7 drug resistant mutation loci ,genotype B and C were detected by using the PCR‐LDR method .The obtained data were analyzed by using the SPSS17 .0 statistical software .Results (1) Genotype C was the main genotype in two areas (Shanghai 79 .3% ,Lanzhou 81 .4% ) .(2) The mutation rate in Shanghai was higher than that in Lanzhou with statistical difference (48 .9%vs .35 .3% ,P<0 .01) .Lamivudine resistance mutation was predominant in all the mutations of two areas ,accounting for 75 .7%(153/202 ;Shanghai 68/92 ,Lanzhou 85/110);the difference in lamivudine resisrtance mutation loci had statistical difference be‐tween the two areas ;while the adefovir resistance loci was dominated by rtA181T/V ,the area difference had no statistical signifi‐cance(P>0 .05) .(3) The drug resistance mutation had no correlation with genotype .The C genotype mutation rate in Shanghai was higher than that in Lanzhou ,the difference was statistically significant (49 .0% vs .35 .4% ,P<0 .05 ) .(4) 4 kinds of drug re‐sistance rate had no correlation with genotype .Conclusion The genotype distributions are similar in two areas ;Shanghai has a higher drug resistance rate than Lanzhou ;the drug resistance occurrence has no relation with drug resistance type and genotype .
