Expression of TBX1 Gene in Kidney Tissues in Patients with Clear Cell Renal Cell Carcinoma and Its Clinical Significance

VernacularTitle:TBX1基因在透明细胞型肾细胞癌组织中的表达及意义
Author: Hongkun JIANG ; Hongyu TIAN ; Yanping GUO ; Yan WANG ; Yuxi ZHANG ; Hong JIANG
Publication Type:Journal Article
Keywords: TBX1; clear cell renal cell carcinoma; RT-PCR; Western blot
From: Journal of China Medical University 2016;45(8):692-694,703
CountryChina
Language:Chinese
Abstract: Objective To analyze the expression of TBX1 gene in kidney tissues in patients with clear cell renal cell carcinoma(ccRCC)and in?vestigate its molecular genetics mechanism during the tumor development. Methods Real?time quantitative polymerase chain reaction(qRT?PCR)was used to detect the expression of TBX1 mRNA in 12 cases of clear cell renal cell carcinoma tissues and the corresponding normal kidney tissues adjacent to carcinoma .The protein expression of TBX1 was assayed by Western blot in both groups. Results Both TBX1 mRNA level and the protein level were significantly up?regulated in ccRCC tissues compare to those in normal kidney tissues adjacent to carcinoma(all P<0.05). Conclusion Over?expression of TBX1 gene might be a potentially pathogenic mechanism of ccRCC.